The use of the letter q to refer to the intercepts of linear features with an area probe originates from the german term querschnitt originally used by weibel in his pioneering work involving the application of stereological methods to biological tissue weibel 1979. Numerous stereological methods have been developed and published, each being specific for different histological tasks. Morphometric analyses are commonly performed on organisms, and are useful in analyzing their fossil record, the impact of mutations on shape. In vivo stereological assessment of human cerebellar volume. The incorporation of data archiving permits easier access to previous studies, as well as the sharing of stereological findings and their related maps throughout the scientific community. This site is dedicated to helping researchers understand the principles of designbased stereology and its advantages over less sophisticated approaches in quantitative histology. The comparison of selected mesophyll parameters was conducted to evaluate the effect of simulated acid rain on current norway spruce needles. The segmental morphometric properties of the horse cervical. Shtereom i simple windowsr based software for stereology. Enter your mobile number or email address below and well send you a link to download the free kindle app. Practical applications of morphometric analysis in toxicology. Using these two stereological techniques, the mean glomerular volume and the total glomerular number per kidney were determined in j 6 kidneys of 8 rats. Stereological tools in biomedical research 471 table i morphometry vs. Methods defined in this technique are essential for proper understanding of the structure of tissue, cells, cellular organelles, and other particles in the body of living organisms.
Stereological techniques are not limited to the biological sciences. Both methods were used in a modified way to correct for variations in section thickness fractionator and to reduce the length of the measuring process weibelgomez, respectively. Methods both eyes of 10 monkeys macaca fascicularis with laserinduced glaucoma in the right eye were studied. This has caused the method of construction to be rethought as well as methods of restoration. Quantification of myocardial fibrosis by digital image. A computed tomography algorithm effectively reconstructs. There were significant improvements in aerobic fitness vo 2 max, insulin sensitivity, and fat oxidation after an overnight fast, as reflected in a decrease in fasting rq.
Novel efficient methods for measuring mesophyll anatomical. Mar 01, 2007 stereology has been earlier defined by wiebel 1970 to be. In despite of the argument about nomenclature morphometry and stereology could denominate the same method aherne and dunnill 1982 most authors consider morphometry a twodimensional quantitative method that uses a caliper mainly a caliper micrometer, a gauge with a calibrated micrometer screw for the measurement of thin objects under microscopic observation. Stereological methods have been adapted to biological samples and are comprehensively described by weibel. The aim of this study was to compare the accuracy of digital image analysis tools and the. Sep 23, 2016 the large size of human tissues requires a practical stereological approach to perform a comprehensive analysis of the whole organ. Stereology the definitive source for stereology information. Then you can start reading kindle books on your smartphone, tablet, or computer no kindle device required. Stereology some systems have been announced to do automatic stereology like c. Objective to assess the relationship between in vivo measurements of optic disc topography and histomorphometric measurements of optic nerve fiber number in glaucoma.
Use the link below to share a fulltext version of this article with your friends and colleagues. Whereas a polished rock surface is really a 2dobject, tissue sections will be 3d by their translucency. An official research policy statement of the american. Biological sciences chemical weapons analysis design and construction. Automated segmentation and morphometry of cell and tissue. The opportunities of confocal microscopy applied to morphometry of microscopical structures are presented and demonstrated on stereological methods based on evaluation of optical sections within a.
Mar 15, 2010 besides biological variation, parameters tested included two variations of stereological methods unbiased counting frame versus 2ddisector, two sampling schemes few large versus frequent small sampling boxes, and workstations with varying degrees of sophistication. Automated segmentation and morphometry of cell and tissue structures. The text by misell 1978 was written prior to the currently available software, but deals extensively with the principles and methods for image enhancement and analysis not confined to computers. Changes induced by physical activity and weight loss in the. The objective of the present study was to determine the morphometric features of the csc segments in the horse and possible relationships among the morphometric features. In response to the intervention, there were substantial reductions in weight, bmi, and fat mass. A comparison of methods, ajp lung cellular and molecular physiology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Software currently in active development the following software is in betatesting phase and as such it may still contain bugs relevant for proper program function. All estimates were validated against exhaustive counts of the same nerve. Alterations of mouse lung tissue dimensions during. Some of the stereological systems now integrate spatial mapping with stereological analyses to provide more comprehensive methods to analyze brain tissue. We will consider how computers are being used to solve several problems of data management and to identify software packages that currently offer new and improved strategies for biological stereology. Although the cervical spinal cord csc of the horse has particular importance in diseases of cns, there is very little information about its segmental morphometry. Characterization of these quantitative changes provides data that is useful not only for making a. We have developed a method to quantitatively analyze the whole. The segmented csc from five mature animals was used. Stereological procedures which allow the assessment of volume percentages, surfaces, lengths, numbers, and diameters are discussed in the light of dimension reduction. Stereology is a completely different enterprise from computed tomography. Is length an appropriate estimator to characterize pulmonary. Unbiased stereological methods were used to evaluate the adrenal gland morphology. Stereology is a powerful, efficient, and reliable set of methods for analyzing the morphology of the lung quantitatively ochs, 2006.
Aug 27, 2009 by means of the cast software visiopharm, denmark, the stereological probes points, lines, and counting frames were superimposed onto the video images of the tissue sections viewed on the monitor. Software for morphometry images of biological drugs a powerful universal tool, which allows directly to control the process of inputting images, scanning products, as well as methods to automate routine image analysis of biological products and samples. Section 1 gives an overview of stereology, sections 2 point counting programs and 3 digitizing programs describe software currently being used for collecting raw data and estimating simple stereological parameters, and section 4 includes a discussion of several specialpurpose programs that offer access to some of the more advanced methods. Stereological analyses of the whole human pancreas. Calibration of the stereological estimation of the number of. The statistical and mathematical principles used in stereology guarantee that no assumptionsor biasare introduced in the analysis. Application of morphometric and stereological techniques on analysis and modelling of the avian lung, new insights into morphometry studies, pere m. Computer programs for biological stereology springerlink. Much of their practical application today is in the field of metallurgy and modifications of the techniques are used in ecology, geography, and earth sciences. Gene, cell and tissue basic application of stereology in.
Tips for studies with quantitative morphology morphometry. This term is widely used in the stereological literature, which we have made an effort to respect in order to support a standardized terminology. Stereological studies demonstrated that the sand was obtained from dunes facing the bays. Actually, they were first devised as a method of analyzing the mineral composition of various rocks. Application of morphometric and stereological techniques on.
When correctly implemented, the sensitivity and efficiency of stereological methods ensures accurate estimates with a fixed and predetermined precision. Everyday low prices and free delivery on eligible orders. Morphometrics from greek morphe, shape, form, and metria, measurement or morphometry refers to the quantitative analysis of form, a concept that encompasses size and shape. One of the wellknown exceptions is imagej software 1, which is a public domain java image. Using these methods, differences can be demonstrated which are missed with the routine subjective methods. They are recommended reading prior to embarking upon a project requiring morphometric or stereologic analysis methods. Despite already available digital methods, the pathologists visual score is still widely considered as ground truth and used as a primary method in histomorphometric evaluations. Morphometric applications in anatomic pathology, human. The optical fractionator technique to estimate cell numbers. Shtereom i is a simple windowsbased software for stereological estimation.
The application of stereological methods for estimating. Practical methods for biological morphometry vol 1 by weibel, ewald r. The sections were evaluated using cavalieris method and volume fraction approach. Results for stereological parameters of the norway spruce needles and their mesophyll anatomical structure obtained using this studys novel stereological methods are shown in table 1. Most of stereological software is just a part of multifunctional software packages and, therefore, could incur very significant expenses that can interfere with the widespread use of computerized stereological tools. In despite of the argument about nomenclature morphometry and stereology could denominate the same method aherne and dunnill 1982 most authors consider morphometry a twodimensional. In the present report, we apply this stereological method to evaluate the effects of age and gender on human cerebellar volume. The set of stereological morphometric grids macros for imagej.
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